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prs426  (ATCC)


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    ATCC prs426
    Prs426, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+no+77107/pRS426+phagemid%3B+in+E%2E+coli/pmc12406275-23-0-2
    Average 93 stars, based on 20 article reviews
    prs426 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 166) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88).

    Article Title: Methods and systems for removing undissolved solids prior to extractive fermentation in the production of butanol
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 73) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. Deletion of NAD-dependent glycerol 3-phosphate dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 130) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Production of alcohol esters and in situ product removal during alcohol fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 151) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Production of alcohol esters and in situ product removal during alcohol fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 151) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. The vector is pUC19 based and contains the sequence of the URA3 gene from Saccharomyces cerevisiae CEN.PK 113-7D situated within a multiple cloning site (MCS). pUC19 contains the pMB1 replicon and a gene coding for beta-lactamase for replication and selection in Escherichia coli.

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. Deletion of NAD-Dependent Glycerol 3-Phosphate Dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England Biolabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 83) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 142) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 145) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Marker:

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 166) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88).

    Article Title: Methods and systems for removing undissolved solids prior to extractive fermentation in the production of butanol
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 73) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. Deletion of NAD-dependent glycerol 3-phosphate dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 130) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Production of alcohol esters and in situ product removal during alcohol fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 151) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Production of alcohol esters and in situ product removal during alcohol fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 151) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. The vector is pUC19 based and contains the sequence of the URA3 gene from Saccharomyces cerevisiae CEN.PK 113-7D situated within a multiple cloning site (MCS). pUC19 contains the pMB1 replicon and a gene coding for beta-lactamase for replication and selection in Escherichia coli.

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. Deletion of NAD-Dependent Glycerol 3-Phosphate Dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England Biolabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 83) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 142) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. GPD2 Deletion To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 145) was PCR-amplified using loxP-URA3-loxP (SEQ ID NO: 68) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. .. PCR was done using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9).

    Plasmid Preparation:

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. Deletion of NAD-dependent glycerol 3-phosphate dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 130) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. Deletion of NAD-Dependent Glycerol 3-Phosphate Dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England Biolabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 83) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Generated:

    Article Title: Extraction solvents derived from oil for alcohol removal in extractive fermentation
    Article Snippet: .. Deletion of NAD-dependent glycerol 3-phosphate dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England BioLabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 8 and 9) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 130) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..

    Article Title: Use of thiamine and nicotine adenine dinucleotide for butanol production
    Article Snippet: .. Deletion of NAD-Dependent Glycerol 3-Phosphate Dehydrogenase A gpd2::loxP-URA3-loxP cassette was PCR-amplified from pUC19::loxP-URA3-loxP plasmid template using Phusion® DNA polymerase (New England Biolabs Inc., Ipswich, Mass.) and primers LA512 and LA513 (SEQ ID NOs: 87 and 88) which generated a ̃1.6 kb PCR product. pUC19::loxP-URA3-loxP (SEQ ID NO: 83) contains the URA3 marker from (ATCC No. 77107) flanked by loxP recombinase sites. ..



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